Mycobacteriophage-repressor-mediated immunity as a selectable genetic marker: Adephagia and BPs repressor selection
نویسندگان
چکیده
منابع مشابه
An Unusual Phage Repressor Encoded by Mycobacteriophage BPs
Temperate bacteriophages express transcription repressors that maintain lysogeny by down-regulating lytic promoters and confer superinfection immunity. Repressor regulation is critical to the outcome of infection-lysogenic or lytic growth-as well as prophage induction into lytic replication. Mycobacteriophage BPs and its relatives use an unusual integration-dependent immunity system in which th...
متن کاملTranscriptional silencing by the mycobacteriophage L5 repressor.
The success of a temperate bacteriophage is dependent upon its ability to completely shut down expression of its lytic genes during lysogenic growth. Mycobacteriophage L5 accomplishes this by an atypical phage repressor, gp71, which binds to multiple asymmetric DNA sites. L5 gp71 regulates transcription initiation at an early lytic promoter, Pleft, but also affects downstream gene expression at...
متن کامل17-P038 Sox3 as a transcriptional repressor
immediate early step in PrE specification. We show that the fraction cells present with in this state is influenced by factors that both promote and suppress primitive PrE differentiation, however conditions that support ES cell self-renewal prevent their progression into endoderm differentiation and support an equilibrium between this state and at least one other that resembles the inner cell ...
متن کاملCloning and sequencing analysis of the repressor gene of temperate mycobacteriophage L1.
The wild-type and temperature-sensitive (ts) repressor genes were cloned from the temperate mycobacteriophage L1 and its mutant L1cIts391, respectively. A sequencing analysis revealed that the 131st proline residue of the wild-type repressor was changed to leucine in the ts mutant repressor. The 100% identity that was discovered between the two DNA regions of phages L1 and L5, carrying the same...
متن کاملNeomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants.
The antibiotic G418 was shown to be an effective inhibitor of vaccinia virus replication when an appropriate concentration of it was added to cell monolayers 48 h before infection. Genetic engineering techniques were used in concert with DNA transfection protocols to construct vaccinia virus recombinants containing the neomycin resistance gene (neo) from transposon Tn5. These recombinants conta...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbiology
سال: 2015
ISSN: 1350-0872,1465-2080
DOI: 10.1099/mic.0.000120